BAX^G67R and BAX^G108V have lost cell death-promoting activity. (A) Western blot analysis using anti-BAX polyclonal antiserum N20 on lysates of FL5.12-BCL-2 cells (Hygro) or HA-BAXα, HA-BAX^G67R, or HA-BAX^G108Vstably transfected clones. (B) Western blot analysis of the same lysates as in (A) using antihuman BCL-2 MoAb 6C8. (C) Viability assay. Transfected clones described in (A) were deprived of IL-3 and viability was determined by trypan blue exclusion at 0, 0.5, 1, 2, 3, 4, and 5 days after IL-3 withdrawal and plotted as the mean percentage of survival ± SEM. (D) Western blot analysis using anti-Bax polyclonal antiserum N20 on lysates of FL5.12 cells (Neo) or HA-BAX, HA-BAX^G67R, or HA-BAX^G108V stably transfected clones. (E) Viability assay. Transfected clones described in (D) were deprived of IL-3 and viability was determined by trypan blue exclusion at 0, 12, 16, 20, 24, 36, and 48 hours after IL-3 deprivation and plotted as the mean percentage of survival ± SEM.