SDS-PAGE gel (A) and Western blot (B) of rhAT (lane 1), rhAT assay standard (lane 2), and phAT (lane 3). Molecular weight markers are shown on the silver stained gel (A). Twenty micrograms of protein were applied to each sample lane (lanes 1, 2, and 3). The SDS-PAGE gel (A) was developed with the Morrissey silver stain, and the Western blot (B) was developed with a sheep antihuman AT-HRP antibody (SeroTec) and color development was the ECL system (Amersham).