Homing of T and B cells into PLN, PP, and spleen in vivo. T and B cells were enriched from spleen of DDD/1-+/+, labeled with BCECF-AM (Dojindo) and 1 × 10⁷ of the cells were intravenously injected. Fluorescence-positive cells in PLN, PP, and spleen of DDD/1-+/+ (filled column) or DDD/1-plt/plt (crosshatched column) were detected with flow cytometry at 48 hours after the injection. Cell number was calculated from the total cell number in each tissue and percentage of fluorescence-positive cells. Mean and SD from two mice is indicated. *P < .072, ** P < .05, *** P < .001.