Expression of L-selectin and CD44 on T-cell surface. Lymphocytes from LN, PP, and spleen of DDD/1-+/+ (dotted line) or DDD/1-plt/plt (solid line) were stained with FITC-conjugated H57-597 (anti-Cβ TCR) and biotinylated MEL-14 (anti–L-selectin) or KM201 (anti-CD44) MoAb followed by phycoerythrin-conjugated streptavidin (Becton Dickinson). The stained cells were analyzed in a FACScan (Becton Dickinson). T cells expressing TCR were gated and their expression of L-selectin or CD44 molecules (bold line) were analyzed. Cells stained only with FITC-conjugated H57-597 and phycoerythrin-conjugated streptavidin as a negative control (fine line) were also shown. Each line indicates the result as follows: bold dotted line: L-selectin or CD44 expression in +/+; bold solid line: L-selectin or CD44 expression in plt/plt; fine dotted line: negative control in +/+; fine solid line: negative control in plt/plt. The percentage of T cells expressing L-selectin or highly expressing CD44 in +/+ orplt/plt was indicated in the each panel. Reproducible results were obtained from three independent similar analyses.