![Fig. 1. Coagulation in normal and hemophilia A blood, with and without replacement. Using 25 pmol/L TF, coagulation was initiated in normal and hemophilia A blood (see the Materials and Methods and Rand et al34) with and without recombinantfactor VIII replacement (1 U/mL blood). Smooth curves have been drawn by hand through the points to approximate the data. (A) Time courses for TAT in normal blood (•), hemophilia A blood (patient A1; ○), and hemophilia A blood with recombinant factor VIII (□). Each point on the normal curve represents an average TAT from 14 experiments on four normal subjects (with error bars, SEM). Average clot time for the composite normal curve is 4.0 ± 0.2 minutes (arrow a). Clotting in factor VIII-deficient blood occurred at 6.5 minutes (arrow c), which was shortened to 4.1 minutes with factor VIII replacement (arrow b); a control tube without TF in the factor VIII-deficient experiment did not clot (>20.6 minutes), and the replacement control clotted at 17.8 minutes. (B) FPA generation in normal blood, factor VIII-deficient blood, and factor VIII-deficient blood with replacement (symbols as in [A]). The normal curve represents the averaged results from nine experiments performed on two individuals, with error bars (SEM). The average clot time for the normal profile was 4.1 ± 0.2 (SEM) minutes (arrow a), with the other clot times as in (A). (C) Osteonectin release was measured to examine platelet activation (symbols as in [A]). The normal curve is constructed from blood taken from a single normal donor (clot time = 4.1 minutes, arrow a) drawn contemporaneously with the factor VIII-deficient patient. Other clot times and symbols are as in (A). (D) After analysis of factor V activation by immunoblotting, profiles were constructed by densitometric analysis as in the Materials and Methods. Time courses are given for formation of the heavy (▪, □) and light chains (•, ○) of factor Va (factor VaHCand VaLC), with factor VIII replacement (solid symbols) and without (open symbols). For clarity, the normal profile is omitted, but is similar to the profile with factor VIII replacement. Clot times are 4.1 minutes (hemophilia A with factor VIII replacement, arrow a) and 6.5 minutes (hemophilia A, arrow b).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/12/10.1182_blood.v91.12.4581/4/blod41212001x.jpeg?Expires=1735534176&Signature=Kb-YYL43j5KidLAW~XjWSni-H0AyoIt1U0smJiTB3jvRKGTFTd~awtg8v6TiD85Qjc42M8~SnSlnuf9LFOkNqaeKtamo9c8KocYBuCSCClxUtcjwnuJiLuMtbGfPGqbZO2FbXOjErCrFY7P90XLNFGA6ToQOEM47zs642PPXj4t1UJoTj0YeCh9ut6-niH8RPGGKUj5GUUwUS4jKHqaM~r5CN9K4rGW8JJEza9JMtFsYAkIQcPKlUnGcwEu6nsF6vY92N9xHQyETZiqhgU8~2B4F0e27EYl1kD4s5jLrHV8Ws-Er-~0Y0FOdlODzrA6p9-5XhkSQB~R7zXOiny7y-Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Coagulation in normal and hemophilia A blood, with and without replacement. Using 25 pmol/L TF, coagulation was initiated in normal and hemophilia A blood (see the Materials and Methods and Rand et al34) with and without recombinantfactor VIII replacement (1 U/mL blood). Smooth curves have been drawn by hand through the points to approximate the data. (A) Time courses for TAT in normal blood (•), hemophilia A blood (patient A1; ○), and hemophilia A blood with recombinant factor VIII (□). Each point on the normal curve represents an average TAT from 14 experiments on four normal subjects (with error bars, SEM). Average clot time for the composite normal curve is 4.0 ± 0.2 minutes (arrow a). Clotting in factor VIII-deficient blood occurred at 6.5 minutes (arrow c), which was shortened to 4.1 minutes with factor VIII replacement (arrow b); a control tube without TF in the factor VIII-deficient experiment did not clot (>20.6 minutes), and the replacement control clotted at 17.8 minutes. (B) FPA generation in normal blood, factor VIII-deficient blood, and factor VIII-deficient blood with replacement (symbols as in [A]). The normal curve represents the averaged results from nine experiments performed on two individuals, with error bars (SEM). The average clot time for the normal profile was 4.1 ± 0.2 (SEM) minutes (arrow a), with the other clot times as in (A). (C) Osteonectin release was measured to examine platelet activation (symbols as in [A]). The normal curve is constructed from blood taken from a single normal donor (clot time = 4.1 minutes, arrow a) drawn contemporaneously with the factor VIII-deficient patient. Other clot times and symbols are as in (A). (D) After analysis of factor V activation by immunoblotting, profiles were constructed by densitometric analysis as in the Materials and Methods. Time courses are given for formation of the heavy (▪, □) and light chains (•, ○) of factor Va (factor VaHCand VaLC), with factor VIII replacement (solid symbols) and without (open symbols). For clarity, the normal profile is omitted, but is similar to the profile with factor VIII replacement. Clot times are 4.1 minutes (hemophilia A with factor VIII replacement, arrow a) and 6.5 minutes (hemophilia A, arrow b).
