Fig. 3.
Localization of sPLA2 in human eosinophils after incubation of the cells with STZ for 10 minutes. (A) Ultrathin cryosections were incubated as described in the legend to Fig 2. The micrograph shows several STZ particles contained in a phagosome that are strongly labeled for sPLA2. Nucleus (n); bar = 500 nm. (B) Micrograph showing some aspecific labeling of the STZ-treated eosinophils incubated with rabbit antimouse IgG and goat antirabbit IgG conjugated to 10-nm gold particles. Some specific granules (g) are seen near the phagosomes. Bar = 500 nm.