Schematic of murine FV cDNA and protein structure. The top bar represents the murine FV protein with the domain boundaries numbered above. The two putative APC cleavage sites at R305 and R504 in murine FV are indicated by arrows. FV domains are labeled as A1-A3, B, and C1-C2. The FV coding sequence (lower bar) was cloned from a C57BL/6J BM cDNA library using the human FV cDNA as probe. The seven unique cDNAs identified are shown as single lines and labeled mFV1-7. The clone labeled “PCR” indicates the DNA fragment obtained through RT-PCR, and “exon 13” is the segment obtained from a genomic library. The major restriction enzyme sites used in the assembly are labeled on the top of the bar [Sac I (781),Apa I (1258), Pst I (1834), Nde I (2073),Kpn I (3976)]. Cla I and Sal I cut the vector sequences adjacent to the FV cDNA.