Analysis of RBCs incubated with FITC-labeled AV after treatment with calcium in the presence of 4 μmol/L ionophore A23187 by fluorescence microscopy. (A) A representative field of this population as observed in bright field and (B) in fluorescence; note the RBC ghost indicated by arrow. (C) A calcium ionophore scrambled spherocytic red cell analyzed by serial optical sections in confocal fluorescent microscopy.