American Society of Hematology

Fig. 4.

$Fig. 4. Cell sorting of GFP-expressing CD34+ cells. Purified CD34+ cells were infected with 100 MOI of AdGFP and analyzed 24 hours after infection for GFP expression. (A) Eleven percent of CD34+ cells expressed GFP, allowing sorting of the cells into GFP+ and GFP− fractions. (B) Reanalysis after cell sorting showed 98% purity of the GFP-expressing population of CD34+ cells. (C) The GFP−fraction was 97% pure.$

Cell sorting of GFP-expressing CD34⁺ cells. Purified CD34⁺ cells were infected with 100 MOI of AdGFP and analyzed 24 hours after infection for GFP expression. (A) Eleven percent of CD34⁺ cells expressed GFP, allowing sorting of the cells into GFP⁺ and GFP⁻ fractions. (B) Reanalysis after cell sorting showed 98% purity of the GFP-expressing population of CD34⁺ cells. (C) The GFP⁻fraction was 97% pure.

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