Fig. 1.
Adult (□) and neonatal (▨) neutrophil adhesion to HUVECs stimulated for 10 minutes with 10−4 mol/L histamine before the addition of neutrophils under shear stress of approximately 2 dynes/cm2. The total number of interacting neutrophils per square millimeter of monolayer includes both rolling and arrested cells. The number of interacting neutrophils was quantified beginning 7.5 minutes after the neutrophil suspension was introduced into the chamber. Neutrophils were left untreated (control) or were preincubated with anti–L-selectin MoAb, DREG 56 (50 μg/mL), or treated with 10 nmol/L fMLP as described. Data are expressed as the mean ± SEM from 7 to 11 experiments. *P < .05 compared with adult control neutrophils.
