Fig. 3.
![Fig. 3. Inhibition of CDK2- and CDC2-associated kinase activities by MPA. Immunoprecipitations were performed using lysates from human T lymphocytes before and after IL-2/PHA-L stimulation and anti-CDK2 (lanes 1 to 7) or anti-CDC2 (lanes 8 to 14) antibodies. Competitive peptides were added to the 48-hour samples to control for the specificity of the assay. The immune complexes were incubated in kinase buffer containing [γ-32P]ATP and histone H1 for 30 minutes, separated on a SDS-PAGE and exposed to radiograph film, as outlined in the Materials and Methods section.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2896.2896_2896_2904/5/blod4082103.jpeg?Expires=1735550489&Signature=vBLUfsgYhtFqFGN81t6Q-wpbyBcHDg6NsOWFOw6lDE13urG85KgZNCAizfs3t2TxDRT2ZtIl~O9YwV6YaY~n1~1RnqLsqbOgNaVb2TLwuic7DopnkItaAWxCEpcgKsUdrD2d71IjbmS3YMP0gnVYFddC9-6onSm~Ymfpy8JAVgScLRcBwwG2jz5Be-FNZ-9JKl6KVhhhAlP~dxwsFQCNJV1QpQoJ5yOZZDiyI7xp7XyFUA-T3cfMTctUS6GZkql3wxTK41f9c8WzPZPlIj-0Znyy2IRxl27FNW9-BhuzcikiPBgrelRHxAf0S0tdavmoU5j01lNJJIvds0SUym50Lw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of CDK2- and CDC2-associated kinase activities by MPA. Immunoprecipitations were performed using lysates from human T lymphocytes before and after IL-2/PHA-L stimulation and anti-CDK2 (lanes 1 to 7) or anti-CDC2 (lanes 8 to 14) antibodies. Competitive peptides were added to the 48-hour samples to control for the specificity of the assay. The immune complexes were incubated in kinase buffer containing [γ-32P]ATP and histone H1 for 30 minutes, separated on a SDS-PAGE and exposed to radiograph film, as outlined in the Materials and Methods section.
