Fig. 4.
![Fig. 4. Immunoprecipitation analysis of [35S]methionine-labeled lysates using anti-p21Waf1 antibody. [35S]methionine-labeled lysates of T lymphocytes stimulated for 24 hours (A) and 42 hours (B) were immunoprecipitated with anti-p21Waf1 antibody (lanes 1, 3, 5, 7, and 9) or with preimmune serum (lanes 2, 4, 6, 8, and 10). Cells were treated with the drugs indicated from time 0 (1 μmol/L MPA, 50 μmol/L guanosine, 100 μmol/L 8-aminoguanosine, 25 μg/mL 5-FU, 10 μmol/L ara-C). The mobility of protein molecular weight standards p21Waf1 and CDK6 are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2896.2896_2896_2904/5/blod4082104a.jpeg?Expires=1735551104&Signature=QVkoKL4-VP7Hxvk97xUmzGjn5ABoSs~Yoeydg~sSeqakcBXLYWBFmeICuvnArYSIQ1RvZebdYZl2yzREi7oYeNEcBLKSl7usgx9wv~S4gsSZkTltzWxvpS1MDFZerXhUmP2zfMPLs6vxan9mihl7Sa2gowUBZDxtgJqIQ25DmFBQR9NRUf5Q4~ws8TbiKDI9nkWL6BwChEkWuCJC3M-sx6I3hsRhoYjXSFa59H3N5GwSpr3jWRMAE9i3rT1LpK93vS94Ns-cqICErnJqOG9hTs4rxOjgIBKyCwZhqsZOMRSl1vB4uk3JUwM~wG~j2eiNqwk2LqWOSc-rxiagGv46gQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation analysis of [35S]methionine-labeled lysates using anti-p21Waf1 antibody. [35S]methionine-labeled lysates of T lymphocytes stimulated for 24 hours (A) and 42 hours (B) were immunoprecipitated with anti-p21Waf1 antibody (lanes 1, 3, 5, 7, and 9) or with preimmune serum (lanes 2, 4, 6, 8, and 10). Cells were treated with the drugs indicated from time 0 (1 μmol/L MPA, 50 μmol/L guanosine, 100 μmol/L 8-aminoguanosine, 25 μg/mL 5-FU, 10 μmol/L ara-C). The mobility of protein molecular weight standards p21Waf1 and CDK6 are indicated.
