Effect of MPA on p27^Kip1 expression. (A) Western blot analysis of lysates immunoprecipitated with p27^Kip1 antibody and detected with the same p27^Kip1 antibody. Resting T lymphocytes (lane 1) were stimulated by the addition of IL-2/PHA-L in absence (lanes 2 to 6) or presence (lanes 7 to 11) of 1 μmol/L MPA for 4, 8, 12, 20, and 24 hours. (B) Western blot analysis of lysates immunoprecipitated with p27^Kip1 antibody and probed with CDK2 antibody. T lymphocytes were stimulated with IL-2/PHA for 24 and 48 hours in absence (lanes 1 to 6) or presence of 1 μmol/L MPA (lanes 7 to 10).