![Fig. 8. Effect of MPA on CDK6 complex formation. (A) [35S]methionine-labeled lysates of stimulated T lymphocytes were immunoprecipitated with anti-CDK6 antibody (stimulation for 42 hours) with or without preincubation with the competing peptide. Cells were stimulated with IL-2/PHA-L in absence (lanes 1 to 2) or presence of 1 μmol/L MPA (lanes 3 to 4) and 50 μmol/L guanosine and 100 μmol/L 8-aminoguanosine (lanes 5 to 6). (B) Western blot analysis of lysates immunoprecipitated with CDK6 antibody and detected with cyclin D3 antibody. Resting T lymphocytes (lane 1) were stimulated with IL-2/PHA-L for 24 and 48 hours in absence (lanes 2 to 3) or presence of 1 μmol/L MPA (lanes 4 to 5) and 50 μmol/L guanosine and 100 μmol/L 8-aminoguanosine (lanes 6 to 7). The mobility of protein molecular weight standards and relevant proteins are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2896.2896_2896_2904/5/blod4082108b.jpeg?Expires=1735550745&Signature=cROeGs9zDmmKPMdMFotRDDvsuPjOfS~MTSJzrlQrOBsOHUV5nQm-YwcIDqrEIfaapzJ~GetDWUZlnfAX5xhN3B-naf1~I59NXPeVN~rsTCj4ObHYnjqD8lguXaLQcuz-FqsugSiAo4ENJpo2b99Y1wat38eE2blRStzrtw8fe~0Ktd4oXmTs9lcMZdWhUVy6SNfkmdJJT~RxZFdX~3lfk78eaCGZ7wh~yM2uoZa99dyx~jQsrGOXi-Of0-u-L2KPZ-Wg6r5DLom6FMSo42fgT7zKJ~cSQ4VvtOY53Y6atXdTfl2vNTQ9DIFt7GoIsyrgrNNhMlhUzwDvjegJykf1mQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of MPA on CDK6 complex formation. (A) [35S]methionine-labeled lysates of stimulated T lymphocytes were immunoprecipitated with anti-CDK6 antibody (stimulation for 42 hours) with or without preincubation with the competing peptide. Cells were stimulated with IL-2/PHA-L in absence (lanes 1 to 2) or presence of 1 μmol/L MPA (lanes 3 to 4) and 50 μmol/L guanosine and 100 μmol/L 8-aminoguanosine (lanes 5 to 6). (B) Western blot analysis of lysates immunoprecipitated with CDK6 antibody and detected with cyclin D3 antibody. Resting T lymphocytes (lane 1) were stimulated with IL-2/PHA-L for 24 and 48 hours in absence (lanes 2 to 3) or presence of 1 μmol/L MPA (lanes 4 to 5) and 50 μmol/L guanosine and 100 μmol/L 8-aminoguanosine (lanes 6 to 7). The mobility of protein molecular weight standards and relevant proteins are indicated.
