Fig. 9.
![Fig. 9. Effect of MPA on CDK6 kinase activity. CDK6-associated kinase activity using affinity-purified antisera against CDK6 and a GST-Rb fusion protein as substrate. Cells were stimulated with IL-2/PHA-L for 24 and 48 hours in absence (lanes 1 to 6) or in presence of 1μmol/L MPA (lanes 7 to 10). Immunoprecipitations were performed with or without incubation with the competing peptide for CDK6. The immune complexes were incubated in kinase buffer containing [γ-32P]ATP and separated by SDS-PAGE and exposed to radiograph film, as outlined in the Materials and Methods section.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2896.2896_2896_2904/5/blod4082109.jpeg?Expires=1735552716&Signature=06~ejvbcLASRdjjCkkiBzlrHBlnXTJTuNHdK6fkMpQYoPeaTf7~ky6ZkkfB0MmL6eJbzURHPL8d3pyEM49UErVHMb3DHRU7aJ6hU-DmjxwYnZkQPBzcGnB022-DTS6rnXbVtuJfhNhWnZ5LpcdJDakXXys6fnQD7WfdwIQjvXq9IuYr9Pt1vbbw-koj02OFqf-4NRWS1HmrYDbA3Z~QhiTSBh~NqQTkdttgQham5n8XOG3pZZyNrcMk0KTAo6Eiv2IF53uUtRamXNnIpsLY5q3I1Iu44jQxaww4do7CPtTkKInsNLxc0F1zdQZzMzTnPxm9gyKEKab6xUzBsck7KDQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of MPA on CDK6 kinase activity. CDK6-associated kinase activity using affinity-purified antisera against CDK6 and a GST-Rb fusion protein as substrate. Cells were stimulated with IL-2/PHA-L for 24 and 48 hours in absence (lanes 1 to 6) or in presence of 1μmol/L MPA (lanes 7 to 10). Immunoprecipitations were performed with or without incubation with the competing peptide for CDK6. The immune complexes were incubated in kinase buffer containing [γ-32P]ATP and separated by SDS-PAGE and exposed to radiograph film, as outlined in the Materials and Methods section.
