Map of the AAV vectors AAV-CMV-F.IX (A) and AAV-EF1α-F.IX (B). ITR, AAV inverted terminal repeat; P (CMV), cytomegalovirus immediate early enhancer/promoter; F.IX, intron I, the coding region of the F.IX cDNA including a portion of intron I (see Materials and Methods); SV40, the simian virus 40 polyadenylation signal; F.IX cr, coding region of the human F.IX cDNA; P (EF1α), promoter of the human polypeptide elongation factor EF1α gene; hGH, polyadenylation signal of the human growth hormone gene.