Levels of human F.IX in plasma of adult C57BL/6 mice as a function of time after portal vein injection of rAAV vector. (A) Mice were injected with 1 × 10¹¹ vector particles of AAV-CMV-F.IX (□) or 6.3 × 10¹⁰ particles of AAV-EF1α-F.IX (▵ and ○). Each line represents an individual animal. (B) Mice were injected with 5.5 × 10¹⁰ particles (▵, n = 4) or 2.7 × 10¹¹ particles (○, n = 4) of AAV-EF1α-F.IX. Each line represents the average of four mice. Vertical bars are the standard deviation.