Northern blot analysis of HIP1 mRNA in various tissues. Blots (Clontech) were probed with with an³²P–end-labeled probe from HIP1 nucleotides 2890 to 2930.20 Exposure time was 12 hours. The lower panels are the same blots stripped and reprobed with actin cDNA. (A) Cell lines. RNA sources were HL60, HELA, K562, MOLT4, Raji, SW480, A549, and G361, designated 1 through 8, respectively. (B) Adult tissue RNA sources are spleen, thymus, prostate, testis, ovary, small intestine, colonic mucosa, and peripheral blood, designated 1 through 8, respectively.