Ezrin and moesin colocalize with CD43 at the uropods of T lymphoblasts stimulated with anti-CD43 MoAb. T lymphoblasts were allowed to adhere to coverslips coated with FN-80 and then stimulated with the anti-CD43 HP2/21 MoAb. (i) Cells were fixed and stained for CD43 (A), ezrin (90/3 pAb) (B), radixin (C), and moesin (38/87 MoAb) (D). Note the redistribution of all three ERM proteins as well as CD43 to cellular uropods. (ii) Cells were double-stained for CD43 (HP2/21 MoAb) (A and C, orange) and for moesin (38/87 MoAb) (B, green) or ezrin (90/3 pAb) (D, green), as described under the Materials and Methods. Arrows point to cellular uropods.