American Society of Hematology

Fig. 4.

$Fig. 4. Association of ezrin and moesin with CD43 in polarized T lymphoblasts. T cells either unstimulated (lanes 1 and 3) or stimulated with anti-CD43 HP2/21 MoAb (lanes 2 and 4) were allowed to adhere to FN80. Cells were then lysed and the soluble fraction was immunoprecipitated with the anti-CD45 RP2/21 (lanes 1 and 2) and anti-CD43 TP1/36 MoAb (lanes 3 and 4). A human recombinant moesin standard was run in the right lanes. Each immunoprecipitate as well as standards were immunoblotted with the antimoesin 95/2 pAb after SDS 7.5% PAGE (A) and reprobed with the anti-ezrin 90/3 pAb (B) as described under the Materials and Methods.$

Association of ezrin and moesin with CD43 in polarized T lymphoblasts. T cells either unstimulated (lanes 1 and 3) or stimulated with anti-CD43 HP2/21 MoAb (lanes 2 and 4) were allowed to adhere to FN80. Cells were then lysed and the soluble fraction was immunoprecipitated with the anti-CD45 RP2/21 (lanes 1 and 2) and anti-CD43 TP1/36 MoAb (lanes 3 and 4). A human recombinant moesin standard was run in the right lanes. Each immunoprecipitate as well as standards were immunoblotted with the antimoesin 95/2 pAb after SDS 7.5% PAGE (A) and reprobed with the anti-ezrin 90/3 pAb (B) as described under the Materials and Methods.

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