American Society of Hematology

Fig. 2.

$Fig. 2. Two-color immunofluorescence demonstrates B-cell expression of CD40L. (A) Unfractionated, peripheral blood cells from patients were analyzed by two-color immunofluorescence flow cytometry immediately after isolation. Shown are three examples including the best case (no. 10), an intermediate case (no. 25), and a low CD40L (CD154) case (no. 28). Each contour plot reflects expression of CD19 on the x-axis, demonstrated with a directly conjugated anti-CD19-FITC MoAb. The plots on the left demonstrate the results after exposure also to a PE-conjugated antibody to CD5, those in the center after exposure to a PE-conjugated antibody to CD3, and those on the right after exposure to a PE-conjugated antibody to CD40L (TRAP clone). The percentage of double-positive cells for each sample is indicated.$

Two-color immunofluorescence demonstrates B-cell expression of CD40L. (A) Unfractionated, peripheral blood cells from patients were analyzed by two-color immunofluorescence flow cytometry immediately after isolation. Shown are three examples including the best case (no. 10), an intermediate case (no. 25), and a low CD40L (CD154) case (no. 28). Each contour plot reflects expression of CD19 on the x-axis, demonstrated with a directly conjugated anti-CD19-FITC MoAb. The plots on the left demonstrate the results after exposure also to a PE-conjugated antibody to CD5, those in the center after exposure to a PE-conjugated antibody to CD3, and those on the right after exposure to a PE-conjugated antibody to CD40L (TRAP clone). The percentage of double-positive cells for each sample is indicated.

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