Eosinophil rolling and firm adhesion visualized by intravital videomicroscopy. Ragweed-sensitized wild-type mice were challenged with an intraperitoneal injection of ragweed. Twenty-four hours after intraperitoneal ragweed allergen challenge, fluorescently labeled eosinophils in the peritoneal microcirculation were visualized in vivo using intravital videomicroscopy. (A) through (F) are sequential videotape images of the same venule (V) and arteriole (A). Blood flow in the venule is from left to right. Three firmly adherent eosinophils (⋆; A) are visualized in the same relative position in (A) through (F), whereas two different eosinophils (eosinophil #1 = ▵; and eosinophil #2 = ⇧) rolling along the venular endothelium are noted in different positions in (A) through (D) (rolling eosinophil #1 = ▵) and (C) through (E) (rolling eosinophil #2 = ⇧).