VEGF mRNA induction by FVII(a). Confluent fibroblasts were incubated with or without 100 nmol/L FVII(a) for the times indicated. Five micrograms of total RNA was analyzed by RT-PCR. (A) Photograph from a representative experiment. Three VEGF mRNA transcripts are seen (180, 312, and 384 bp). The internal standard, mimic (M), is also visible. (B) RNA was quantified was achieved by scanning the Polaroid negative by laser densitometry. The density of the 180-bp band was normalized to the density of the mimic, and the fold induction of VEGF mRNA induced by FVII(a) compared with unstimulated cells at the same time was plotted. Each point represents the mean ± SEM of three experiments. *P < .01 versus unstimulated fibroblasts.