American Society of Hematology

Fig. 1.

$Fig. 1. (A) Migration of unfractionated thymocytes in response to MIP-1β (0.01 to 100 nmol/L), or PHA (0.01 to 100 μg/mL). Each point represents the mean ± SEM for migration from n = 4 experiments performed in duplicate. / (B) Migration of thymocytes from each purified subpopulation in response to MIP-1β. Antibody-labelled cells were sorted on the FACStar Plus cell sorter, as detailed in the Materials and Methods section. Sorted cells were collected from the populations delineated by the gates in the dot-plot. Cells were washed once in PBS, then used immediately for assay of migration. Each point represents the mean ± SEM migration from n = 3 experiments performed in duplicate.$

(A) Migration of unfractionated thymocytes in response to MIP-1β (0.01 to 100 nmol/L), or PHA (0.01 to 100 μg/mL). Each point represents the mean ± SEM for migration from n = 4 experiments performed in duplicate.

(B) Migration of thymocytes from each purified subpopulation in response to MIP-1β. Antibody-labelled cells were sorted on the FACStar Plus cell sorter, as detailed in the Materials and Methods section. Sorted cells were collected from the populations delineated by the gates in the dot-plot. Cells were washed once in PBS, then used immediately for assay of migration. Each point represents the mean ± SEM migration from n = 3 experiments performed in duplicate.

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