American Society of Hematology

Fig. 2.

$Fig. 2. MIP-1β–induced Ca2+ mobilization in unfractionated human thymocytes. Freshly-isolated cells were loaded with 3 mmol/L (final) Indo-1AM and kept in a constantly-stirred cuvette, as detailed in the Materials and Methods section. Stimulation was with 100 nmol/L recombinant human MIP-1β. The inset shows the flux profile obtained in response to 2 μg/mL PHA as a positive control. Flux profiles are representative examples taken from n = 6 experiments.$

MIP-1β–induced Ca²⁺ mobilization in unfractionated human thymocytes. Freshly-isolated cells were loaded with 3 mmol/L (final) Indo-1AM and kept in a constantly-stirred cuvette, as detailed in the Materials and Methods section. Stimulation was with 100 nmol/L recombinant human MIP-1β. The inset shows the flux profile obtained in response to 2 μg/mL PHA as a positive control. Flux profiles are representative examples taken from n = 6 experiments.

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