Differential sensitivity of ceramide- and Fas-triggered cell death to activation of NF-κB. Jurkat cells were transfected with 5 μg of either CMV-RelA or CMV vector, together with 5 μg of pEGFP-N1 (Clontech). Thirty-six hours later, cells were untreated or treated with either C₂-ceramide (5 μmol/L) or anti-Fas antibody CH11 (100 ng/mL). Ceramide-induced cell death was analyzed 6 hours later, whereas Fas-induced cell death were quantitated 14 hours later. Data are the average of duplicates.