Fig. 1.
Experimental protocols. Experimental protocol 1: Female MRL/lpr mice (3 to 4 months of age) with autoimmune diseases were irradiated (8.5 Gy) and transplanted with T-cell–depleted BMCs plus bone graft of B6 mice. Experimental protocol 2: Female MRL/lpr mice with autoimmune diseases were intraperitoneally injected with 100 mg/kg CY and then lethally irradiated (5 Gy × 2 = 10 Gy for 4-hour interval) 1 day later. Four hours after the second irradiation, the bones of the B6 mice, from which the BMCs had been flushed out, were engrafted under the subcutis of the MRL/lpr mice, and the mice received 5 × 107 WBMCs from B6 mice. One day after the first BMT plus bone graft, the mice were further transplanted with B6 WBMCs (5 × 107 cells).
