(A through C) Interphase FISH of patient no. 1 (AML withAF10 and CALM rearrangement, Table 1). Three AF10 signals (green), indicating a split of one YAC signal, and two centromere 10 signals (red) are detectable in (A). In (B), the proximal (green) and the distal CALM YAC (red) are separated, indicating a breakpoint within this genomic region. Two MLL signals (green) and two centromere 11 signals (red) suggest no rearrangement of the MLL region in (C). (D through F) Interphase FISH of patient no. 8 (AML with AF10 and MLL rearrangement). Three AF-10 signals (green), indicating a split of one YAC signal, and two centromere 10 signals (red) are detectable in (D). In (E), the proximal (green) and the distal CALM YAC (red) are colocalized, excluding a rearrangement of this genomic region. In (F), the detection of three MLL signals (green), but only two centromere 11 signals (red) indicate a split of one MLL probe (C).