Flow cytometric analysis on adult lymphocytes. (A) PE-anti-αβTCR and FITC-anti-Cbl double staining on lymphocyte. (B) PE-anti-αβTCR and FITC-anti-Ship double staining on lymphocytes. The αβTCRhi and αβTCRlo populations are gated (indicated in the upper panel as TCRhi and TCRlo), and FITC fluorescence intensity among the gated cell populations are compared by overlay histogram (lower panel). (C) FITC-anti-Ship, PE-anti-CD4, Texas red-anti-CD8 triple-color staining on lymphocytes. CD4+, CD8+, and DP cells are gated as indicated in the upper panel. FITC fluorescence intensity in the gated cell populations, which correspond to the levels ofship expression, is compared in an overlay histogram (lower panel). SP in the lower histogram of panel C represents both CD4+ (solid line) and CD8+ (filled area) cells. Each plot is representative of 5 independent experiments.
