Comparison of effects of pharmacologically diverse antagonists or medium change on the STAT1-pTyr and STAT2-pTyr induced in IFN-sensitive Daudi cells (D_W^S). After cell exposure to IFN-α2a (100 U/mL) for 18 hours, each antagonist was added to the medium for 1 or 2 hours as indicated. Alternatively, the cells were sedimented twice for a complete medium change (wash). Whole cell extracts were analyzed by IP, gel electrophoresis, and 4G10 Western blots. Total latent and phosphorylated STATs were assessed by reprobing of the transfer membranes with the specific anti-STAT antibodies and secondary detection by ECL.