Fig. 8.
![Fig. 8. (A) 125I-Tf binding studies.125I-Tf binding to cells was measured after growth of cells in fresh medium for the times shown. Open columns, S cells; hatched columns, R1 cells. Data shown represent the means ± SE (n = 3). (B) TfR synthesis. Newly synthesized TfRs were labeled with [35S]methionine over a 3-hour or 20-hour pulse as described in the text. The 3-hour pulse was performed on cells after they had been incubated in fresh medium for 24 hours. The 20-hour pulse was performed immediately after the initial plating of confluent cells in fresh medium (0 to 20 hours). The autoradiograph shown is representative of three separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/12/10.1182_blood.v91.12.4686/4/blod41215008w.jpeg?Expires=1735547338&Signature=EG4hJC24tFKEUrERayQKCZk2~eNESoC9bHhTHzmsn-rHSCa9vkP~SUdVoXm3AEfjzSl6k3hjynY3DAwLEVD0IiwsnNHbRlb4E8h6XLJeL~hkOuYwJ9cp4X3g80Kg4818OgWv-1eAdIJ409n-Y-9XO4xn2qPEt-GE~ElDDC8JUzLyzRNdTEfFlnLVQA-hkRsVPAaOOsYBy4xifP6WezrsRrlQfhjRvPOEZ46qxvNYahcExo2hiaj2hr12xKFwGSkNvYYm59lutY2YCwN2M5GyD6ziWv8k~k4e2lTcv84wyREcsw6VLGgX8sqEJzWycpe9rfILzrSFSMShY-~dMWoEjA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
(A) 125I-Tf binding studies.125I-Tf binding to cells was measured after growth of cells in fresh medium for the times shown. Open columns, S cells; hatched columns, R1 cells. Data shown represent the means ± SE (n = 3). (B) TfR synthesis. Newly synthesized TfRs were labeled with [35S]methionine over a 3-hour or 20-hour pulse as described in the text. The 3-hour pulse was performed on cells after they had been incubated in fresh medium for 24 hours. The 20-hour pulse was performed immediately after the initial plating of confluent cells in fresh medium (0 to 20 hours). The autoradiograph shown is representative of three separate experiments.
