Fig. 5.
Effect of ATRA on TF and TNF-α gene transcription in LPS stimulated THP-1 cells. Nuclei were isolated from unstimulated cells, cells stimulated with LPS (10 μg/mL) for 1 hour, and cells treated with ATRA (10−5 mol/L) for 30 minutes before LPS stimulation for 1 hour. The rate of transcription of the TF, TNF-α, and G3PDH gene was determined by nuclear run-on. Vector controls used in the experiment were pSP73 and pGEM3Z. The autoradiogram was exposed for 7 days at −80°C with intensifier screens. Similar results were observed in an independent experiment.
