LOH analysis and comparative multiplex PCR of normal (N), low-grade (LG), and progressed DLCL (HG) components of a selected case (case no. 9). (A) Case no. 9 showing loss of upper alleles at D9S171 and D9S1752 in the DLCL (HG) lanes. (B) Comparative multiplex PCR showing marked diminution in the intensity of the IFNA alleles in the DLCL lanes (HG) in comparison to the normal (N) and the LGFCL (LG) lanes. In addition, the signal intensity of the IFNA alleles is lower in both (HG) lanes in comparison to the D9S200 alleles.