Fig. 8.
![Fig. 8. Fluorescence imaging of [Ca2+]i responses from individual cells after CD3, CD148, and CD3+CD148 crosslinking. Cells previously loaded with fura-2 (2 μmol/L), were incubated with the corresponding antibody (CD3, 10 μg/mL; 14341, 40 μg/mL; CD45, 40 μg/mL) for 30 minutes at 4°C. After washing, cells were plated on Cell-Tak–coated glass coverslips. After establishing baseline values, saturating amount of polyclonal GAM was added to prewarmed samples. [Ca2+]i was measured every 5 seconds for 15 minutes in every cell. Calcium changes are expressed as the percentile change of basal. A total of 20 cells representative of each condition are shown. Each line represents an individual cell.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2800.2800_2800_2809/5/blod4082308.jpeg?Expires=1735559472&Signature=VdThISCYmxNB8NnSLFqAajJVCV2f4li6Xxp73x5Xa4ltAYSuJ78E5wdmj5lsso2f8Mzo9cCL~7-ulq2e16wtszWoSrKOzW~1pZQLvxSt~be8CNzOWca-E8wo24PPhwgyTgtyJpCDVBDfJStSABYtVgsfvBnQ3mQeylD1fWpDrohmDCAIQV3s6bi12oc-E9t7jSBvTus6dma0skAv7hHWylXp4ZNxclliJCraX-hu~rAp0qSTgoygkjNCLF0mjq~1d~blaJkmzeg28uwD0C3Z~oTQyLNs-9NcY0g8lJG79ega5L6nUhYrXIYbUVfSSr4VD~2bpAE9ZuvW71BY2sbUHA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fluorescence imaging of [Ca2+]i responses from individual cells after CD3, CD148, and CD3+CD148 crosslinking. Cells previously loaded with fura-2 (2 μmol/L), were incubated with the corresponding antibody (CD3, 10 μg/mL; 14341, 40 μg/mL; CD45, 40 μg/mL) for 30 minutes at 4°C. After washing, cells were plated on Cell-Tak–coated glass coverslips. After establishing baseline values, saturating amount of polyclonal GAM was added to prewarmed samples. [Ca2+]i was measured every 5 seconds for 15 minutes in every cell. Calcium changes are expressed as the percentile change of basal. A total of 20 cells representative of each condition are shown. Each line represents an individual cell.
