Neutrophilia in the blood, spleen, and bone marrow of infected IFN-γ −/− mice. (A) Peripheral blood (left panels) or spleen cytospins (right panels) from infected IFN-γ +/+ or IFN-γ −/− mice (indicated at right) were stained with leukostat. Note that in the peripheral blood of IFN-γ −/− mice there are large numbers of mature neutrophils as well as less mature band forms and numerous monocytes in this smear (WBC of this mouse was 1.5 × 105/mL). The spleen of IFN-γ −/− also shows cells predominantly of the granulocyte series of various stages of maturity. All figures were photographed through a 40X objective). (B) Bone marrow sections (left panels) or bone marrow cytospins (right panels) from infected IFN-γ +/+ or IFN-γ −/− mice (indicated at right) were stained with H&E (sections) or leukostat (cytospins). The bone marrow of infected IFN-γ +/+ mice displays normal cellularity and populations of the various cell types. The marrow of IFN-γ −/− mice has large foci of granulocyte development and the cell types are largely band forms. Sections were photographed with a 20X objective and cytospins with a 40X objective.
