Isolation of enucleated erythrocytes from cultures by flow cytometry. (A) shows a FACS analysis of a 3-week-old culture initiated from CD34⁺ cells stained with Hoechst 33342 along the X-axis and glycophorin A along the Y-axis. The two distinct populations, Hoechst-negative [Hoechst (−)] and Hoechst-positive [Hoechst (+)] cells were sorted (marked with arrows). (B) and (C) depict the Wright-Giemsa–stained cytospins of the Hoechst (−) and the Hoechst (+) populations, respectively.