Globin chain synthesis by erythrocytes derived in vitro resembles adult pattern of globin synthesis. (A) and (B) show the reverse-phase HPLC analyses of normal umbilical cord blood and adult peripheral blood (representing fetal and adult-type globin production), respectively. (C) shows the same analysis on enucleated erythrocytes generated in vitro from CD34⁺ cells from normal bone marrow and sorted after Hoechst 33342 labeling. The order of appearance of various globin chains (from left to right) is as follows: β-, δ-, α-, Aγ^T-, Gγ-, and Aγ^I-globin. The X-axes represent retention time and the Y-axes represent absorbance.