Fig. 8.
Growth of XG-2 tumors in vitro. Tumors growing in SCID mice injected with XG-2 cells and agonist anti-gp130 MoAbs were put in suspension and the viable cells recovered by centrifugation on Ficoll hypaque. These cells were cultured at a concentration of 2.5 × 105 cells/mL in culture medium supplemented with 10 μg/mL of control murine IgG1 (control), 3 ng/mL of IL-6 (IL-6), or 10 μg/mL of a mixture of the B1 and I2 anti-gp130 MoAbs (5 μg each; anti-gp130 antibodies). Every 5 days, cells were counted and diluted at the initial cell concentration with fresh culture medium and the initial concentration of cytokine or antibodies. Results are the mean cumulative numbers of cells determined on six different culture wells.
