CD34⁺CD33^lo and CD34⁺CD33⁻ postnatal thymocytes cultured with multiple cytokines give rise to phenotypically distinct cell progenies. (A) Sorted CD34⁺CD33^lo and CD34⁺CD33⁻ postnatal thymocytes were reanalyzed for the expression of CD34 and CD33. (B) The correlated expression of CD44 and CD5 was independently analyzed on sorted CD34⁺CD33^lo (left panels) and CD34⁺CD33⁻ (right panels) cells either before (day 0) or after 3 and 5 days of culture with a mixture of IL-7, IL-1α, IL-6, SCF, and GM-CSF. (C) The correlated expression of CD34 and CD33 was analyzed on electronically gated CD44^bright(left panel) and CD44^lo (right panel) cell progenies recovered at day 3 from the multicytokine-supported cultures of CD34⁺CD33^lo thymocytes.