Human LSP1 expression in the stably transfected A7 melanoma cells, A7ML1, in the absence/presence of Zn²⁺. Shown are immunoblots with anti-LSP1 of 50 μg total cellular protein from (P) PMNs, (A) parent A7 melanoma cells alone + 100 μmol/L Zn²⁺ for 6 hours, (B) the A7 melanoma line stably transfected with pMEP vector alone + 100 μmol/L Zn²⁺for 6 hours; (C and D) the A7ML1 cell line stably transfected with pMEP-huLSP1 in the absence (C) and presence (D) of 100 μmol/L Zn²⁺ for 6 hours. MW, molecular weight marker. The immunoblot was developed colorimetrically.