Independence of TRAIL-mediated NFκB activation from caspases. CEM cells were preincubated with LLnL (2.5 μmol/L) or zVAD (50 μmol/L) for 1 hour. TRAIL (0.3 μg/mL) or PMA (50 ng/mL)/Ionomycin (2 μg/mL) (P/I) was added for another 30 minutes. Cells were harvested and EMSA was performed. sc, specific competition with unlabeled oligo. Similar results were obtained in two independent experiments. Similar results were obtained using Jurkat cells.