Fig. 1.
The detection of cells undergoing apoptosis in BM. The mice were injected with 5 × 107 of B6 spleen cells. On day 14 after transfer, the mice were killed to remove their BM cells. Using flow cytometry, the signals of PI, which represent the DNA contents, were examined. The G0/G1 peak was adjusted at channel 100 and subdiploid cells were designated as cells undergoing apoptosis. The numbers in the figure represent the percentage of cells undergoing apoptosis. Four mice were examined in each group and the representative data were shown. *Significant increases versus uninjected control (P < .05).