American Society of Hematology

Fig. 1.

$Fig. 1. Monocytes suppress OKT3-stimulated T-cell proliferation. (A) Control PBMC at fixed numbers (100 × 103) plus varying numbers of autologous control monocytes obtained by counterflow centrifugal elutriation were cultured in flat-bottom 96-well plates precoated with OKT3 antibody at a concentration of 50 ng/mL. Proliferation was measured on day 4 by 3H-thymidine incorporation. Values represent the mean ± SEM from triplicate cultures. (B) Unfractionated G-PBMC (solid line) or CD14-depleted G-PBMC (dashed line) at increasing numbers were cultured in OKT3-coated 96-well plates. Cultures were pulsed and harvested as described above. Results from one of three experiments are shown.$

Monocytes suppress OKT3-stimulated T-cell proliferation. (A) Control PBMC at fixed numbers (100 × 10³) plus varying numbers of autologous control monocytes obtained by counterflow centrifugal elutriation were cultured in flat-bottom 96-well plates precoated with OKT3 antibody at a concentration of 50 ng/mL. Proliferation was measured on day 4 by ³H-thymidine incorporation. Values represent the mean ± SEM from triplicate cultures. (B) Unfractionated G-PBMC (solid line) or CD14-depleted G-PBMC (dashed line) at increasing numbers were cultured in OKT3-coated 96-well plates. Cultures were pulsed and harvested as described above. Results from one of three experiments are shown.

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