American Society of Hematology

Fig. 4.

$Fig. 4. Change of proliferative responsiveness of control and G-PBMC with neutralization of endogenous IL-10 in MLC. In the experiment, 105 (○) or 2 × 105 (•) unfractionated control (n = 6) or G-PBMC (n = 8) responders plus 100 × 103 irradiated (30 Gy) allogeneic PBMC stimulators were cultured in round-bottom 96-well plates. Proliferation was measured on day 5 by 3H-thymidine incorporation as described in Materials and Methods. Values represent the mean change of proliferation in triplicate cultures containing neutralizing antibodies to IL-10 compared with controls. *Only data points derived from paired G-PBMC and control PBMC samples contributed to the P value (n = 6).$

Change of proliferative responsiveness of control and G-PBMC with neutralization of endogenous IL-10 in MLC. In the experiment, 10⁵ (○) or 2 × 10⁵ (•) unfractionated control (n = 6) or G-PBMC (n = 8) responders plus 100 × 10³ irradiated (30 Gy) allogeneic PBMC stimulators were cultured in round-bottom 96-well plates. Proliferation was measured on day 5 by ³H-thymidine incorporation as described in Materials and Methods. Values represent the mean change of proliferation in triplicate cultures containing neutralizing antibodies to IL-10 compared with controls. *Only data points derived from paired G-PBMC and control PBMC samples contributed to the P value (n = 6).

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