American Society of Hematology

Fig. 6.

$Fig. 6. IL-10 production by unfractionated control and G-PBMC and purified control and G-PBMC monocytes. (A) Unfractionated mononuclear cells (1.5 × 106/mL), or (B) purified monocytes (0.5 × 106/mL) were isolated as described in Materials and Methods and cultured in flat-bottom 96-well plates in the presence or absence of LPS at indicated concentrations. Supernatants from triplicate cultures were harvested after 24 hours, pooled, and IL-10 levels were determined by ELISA. (*) Indicates a P value < .05 compared with control.$

IL-10 production by unfractionated control and G-PBMC and purified control and G-PBMC monocytes. (A) Unfractionated mononuclear cells (1.5 × 10⁶/mL), or (B) purified monocytes (0.5 × 10⁶/mL) were isolated as described in Materials and Methods and cultured in flat-bottom 96-well plates in the presence or absence of LPS at indicated concentrations. Supernatants from triplicate cultures were harvested after 24 hours, pooled, and IL-10 levels were determined by ELISA. (*) Indicates a P value < .05 compared with control.

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