Fig. 3.
Effect of ATRA on cellular procoagulants analyzed by chromogenic and immunological assays for TF and CP expressed by S-NB4 cells and the two R-NB4 cell lines, NB4.306 and NB4.007/6, cultured for 96 hours with 10−6 mol/L ATRA or the vehicle. TF functional activity (A) was measured as rate of FX hydrolysis by the TF/FVII complexes in cell lysates. TF antigen (B) was measured in TBT cell extracts by ELISA, using an anti-human TF MoAb. CP functional activity (C) was measured in VB cell extracts by a three-stage chromogenic assay. CP antigen (D) was immunologically identified by a dot-blot assay using a pure anti-CP monoclonal IgG. The results are the mean of at least three experiments. Statistical analysis as in Fig 1. *P < .05, **P < .01.
