Northern analysis of GP Ibα expression in mice administered LPS. As a model of gram-negative sepsis, LPS was administered to mice (colony 32) by intraperitoneal injection (25 mg/kg). As described in Fig 2, total RNA was prepared from treated (+) and control (−) mice. (A) Lanes 1 to 9 correspond to the same organs listed in the legend for Fig 2. Again, a visible GP Ibα transcript is only present in bone marrow (lane 9) with no increase in GP Ibα expression detected in any organ, but an absence of the GP Ibα bone marrow transcript 24 hours post-LPS treatment. (B) The nitrocellulose filter of (A) was rehybridized with an ICAM-1–radiolabeled cDNA fragment and confirmed an inflammatory state in the treated mice with an increase in the lung ICAM-1 mRNA (lane 5).