Fig. 2.
![Fig. 2. Conversion of 5-FC into 5-FU in PBSCs, MCF-7 BCCs, and an 0.1% mixture of MCF-7 cells in PBSCs after exposure to Ad.CMV-CD adenoviral vector. Cells (2.5 × 106) were exposed for 90 minutes to 100 MOI of Ad.CMV-CD and then incubated for 48 hours in a 25-cm2 flask with 10 mL of medium containing 500 μmol/L 5-FC and 40 μCi of [6-3H]5-FC. One milliliter of medium was collected after 24 hours, mixed with an equal amount of ice-cold methanol, and analyzed by HPLC as described in the Materials and Methods. The numbers above each histogram column indicate the total amount of drug in micromoles (either 5-FC or 5-FU) per flask.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/2/10.1182_blood.v92.2.672/5/blod41419002x.jpeg?Expires=1735501454&Signature=vWuS9iKqb8qs~zO5DA8gjy55tMvlVgoKm9lw8dn3VcAvSFKt5EFXiZ7jK3up7jhklSDUeCAMTet640id2dCbLAhJiJuceKF6pJtjQvjl5L~llFdu-HTzA4uy70wijs~UZokLpF7xBp~M-ozU1f7nl3Dh3bsXwA00xbFKSDQklX1c3afT1F2~8erYt30-UBz6fbjHnopCo2DP6UW1E8FOzhDocNY1vYLMyVr1KW9lwgawqhV1B3dgYXTwoGpUyH~lJnnl0wqBgWISaIvBNxFDtECrxq3o4epYG2CYq9OsWw6mBsjtnchQGtzZID9NT43MjvfAodw74QKizWj827A1Hg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Conversion of 5-FC into 5-FU in PBSCs, MCF-7 BCCs, and an 0.1% mixture of MCF-7 cells in PBSCs after exposure to Ad.CMV-CD adenoviral vector. Cells (2.5 × 106) were exposed for 90 minutes to 100 MOI of Ad.CMV-CD and then incubated for 48 hours in a 25-cm2 flask with 10 mL of medium containing 500 μmol/L 5-FC and 40 μCi of [6-3H]5-FC. One milliliter of medium was collected after 24 hours, mixed with an equal amount of ice-cold methanol, and analyzed by HPLC as described in the Materials and Methods. The numbers above each histogram column indicate the total amount of drug in micromoles (either 5-FC or 5-FU) per flask.
