Infectivity of different human BCC lines by the β-galactosidase Ad.CMV adenoviral vector. BCCs were exposed in suspension for 90 minutes (red line) or 24 hours (blue line) to 100 MOI of the Ad.CMV-βgal vector. Twenty-four hours later, the cells were washed with PBS, stained with FDG, and analyzed by flow cytometry as described in the Materials and Methods. The profile defined by the black line is the control in the absence of virus. The red and the blue profiles are from samples exposed to the vector for 90 minutes and 24 hours, respectively.