Expression of c-fms mRNA and nonspecific esterase activity in CD11b^−/dullCD11c⁺, CD11b^+hiCD11c⁺ DC precursors, and their derived DCs. (A) Expression of c-fms mRNA was examined in the indicated cells using RT-PCR. Total RNAs were extracted from 1 × 10⁵ indicated cells. The β-actin transcripts were used as control. Lane 1, 1-kb DNA ladder; 2, Lin⁻c-kit⁺ HPC; 3, CD11b^−/dullCD11c⁺ precursor; 4, CD11b^−/dullCD11c⁺ mature DC derived from CD11b^−/dullCD11c⁺ precursors; 5, CD11b^+hiCD11c⁺ precursor; 6, CD11b^−/dullCD11c⁺ mature DC derived from CD11b^+hiCD11c⁺ precursors; 7, macrophage derived from M-CSF–induced CD11b^+hiCD11c⁺ DC precursors. (B through F) The cultured cells were sorted and processed for nonspecific esterase staining. (B) CD11b^−/dullCD11c⁺precursors; (C) CD11b^−/dullCD11c⁺precursor-derived CD11b^−/dullCD11c⁺mature DCs; (D) CD11b^+hiCD11c⁺ DC precursors; (E) CD11b^+hiCD11c⁺ DC precursor-derived CD11b^−/dullCD11c⁺ mature DCs; (F) macrophages derived from M-CSF–induced CD11b^+hiCD11c⁺ DC precursors. Original magnification ×400.